JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
/
형질감염된 HeLa 세포에서 mtDNA 및 유전자 발현 수준을 측정하기 위한 정량적 이미지 분석
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells
Please note that all translations are automatically generated.
Click here for the English version.
형질감염된 HeLa 세포에서 mtDNA 및 유전자 발현 수준을 측정하기 위한 정량적 이미지 분석
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
DOI:
10.3791/200044-v
•
03:02 min
•
May 05, 2023
•
Lukasz S. Borowski
2
,
Karolina Kasztelan
3
,
Jolanta Czerwinska-Kostrzewska
2
,
Roman J. Szczesny
1
Faculty of Biology, Institute of Genetics and Biotechnology
,
University of Warsaw
,
2
Institute of Biochemistry and Biophysics
,
Polish Academy of Sciences
,
3
International Institute of Molecular and Cell Biology in Warsaw
Tags
Quantitative Image Analysis
MtDNA Levels
Gene Expression
Transfected HeLa Cells
Fluorescence Imaging
Background Correction
Image Segmentation
BrdU
Mitochondrial DNA
Quantitative Real-time PCR
Dideoxycytidine
DDC
TFAM
TWINKLE Helicase
POLG
Mitochondrial DNA Polymerase Gamma
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/200044?article_type=v
미토콘드리아 DNA 합성 및 분포의 고처리량 이미지 기반 정량화
check_url/200044?article_type=v
미토콘드리아 DNA 합성을 위한 siRNA 혼합물의 제조
check_url/200044?article_type=v
HeLa 세포에서 세포 형질주입 및 5-브로모-2'-데옥시유리딘(BrdU) 혼입
check_url/200044?article_type=v
HeLa 세포에서 미토콘드리아 DNA의 5-브로모-2'-데옥시우리딘(BrdU) 표지
check_url/200044?article_type=v
Hoechst 33342를 이용한 HeLa 세포 고정 및 차단
check_url/200044?article_type=v
mtDNA 분포를 모니터링하기 위해 형질주입된 HeLa 세포에 1차 및 2차 항체 추가
check_url/200044?article_type=v
형질감염된 HeLa 세포에서 mtDNA 및 유전자 발현 수준을 측정하기 위한 정량적 이미지 분석
Read Article
