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Journal
/
Cancer Research
/
Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment
/
Multiplex Immunofluorescence Staining and Image Analysis
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Multiplex Immunofluorescence Staining and Image Analysis
Multiplex Immunofluorescence Staining and Image Analysis
Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment
DOI:
10.3791/200119-v
•
04:44 min
•
June 02, 2023
•
Nicolas Huyghe
,
Elena Benidovskaya
,
Simon Beyaert
,
Aurélie Daumerie
,
Finoula Maestre Osorio
,
Frank Aboubakar Nana
3
,
Caroline Bouzin
,
Marc Van den Eynde
5
1
Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO
,
Université Catholique de Louvain (UCLouvain)
,
2
Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU)
,
Université Catholique de Louvain (UCLouvain)
,
3
Division of Pneumology, Cliniques Universitaires St-Luc
,
Université Catholique de Louvain (UCLouvain)
,
4
IREC Imaging Platform
,
Université Catholique de Louvain (UCLouvain)
,
5
Institut Roi Albert II, Department of Medical Oncology and Gastroenterology
,
Cliniques Universitaires St-Luc
Tags
Multiplex Immunofluorescence
Deparaffinization
Endogenous Peroxidase Inhibition
Citrate Buffer
Microwave
Tris-buffered Saline
Tween 20
BSA
Primary Antibody
Poly-HRP Secondary Antibody
Tyramide Reagent
Pan-Cytokeratin Antibody
BisBenzimide
Fluorescent Staining
Image Analysis
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Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
check_url/200119?article_type=v
Multiplex Immunofluorescence Staining and Image Analysis
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