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Journal
/
Cancer Research
/
Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment
/
マルチプレックス免疫蛍光染色と画像解析
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Multiplex Immunofluorescence Staining and Image Analysis
Please note that all translations are automatically generated.
Click here for the English version.
マルチプレックス免疫蛍光染色と画像解析
Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment
DOI:
10.3791/200119-v
•
04:44 min
•
June 02, 2023
•
Nicolas Huyghe
,
Elena Benidovskaya
,
Simon Beyaert
,
Aurélie Daumerie
,
Finoula Maestre Osorio
,
Frank Aboubakar Nana
3
,
Caroline Bouzin
,
Marc Van den Eynde
5
1
Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO
,
Université Catholique de Louvain (UCLouvain)
,
2
Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU)
,
Université Catholique de Louvain (UCLouvain)
,
3
Division of Pneumology, Cliniques Universitaires St-Luc
,
Université Catholique de Louvain (UCLouvain)
,
4
IREC Imaging Platform
,
Université Catholique de Louvain (UCLouvain)
,
5
Institut Roi Albert II, Department of Medical Oncology and Gastroenterology
,
Cliniques Universitaires St-Luc
Tags
Multiplex Immunofluorescence
Deparaffinization
Endogenous Peroxidase Inhibition
Citrate Buffer
Microwave
Tris-buffered Saline
Tween 20
BSA
Primary Antibody
Poly-HRP Secondary Antibody
Tyramide Reagent
Pan-Cytokeratin Antibody
BisBenzimide
Fluorescent Staining
Image Analysis
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腫瘍微小環境の臨床的および生物学的評価のための空間画像解析と組み合わせたマルチプレックス免疫蛍光法
マルチプレックス免疫蛍光染色と画像解析
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