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Journal
/
Biology
/
Investigating ATG9A Localization in Cells
/
Endogenous Staining of ATG9A from HEK293A Cells
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Endogenous Staining of ATG9A from HEK293A Cells
Endogenous Staining of ATG9A from HEK293A Cells
Investigating ATG9A Localization in Cells
DOI:
10.3791/200171-v
•
03:19 min
•
June 16, 2023
•
Alexander R. van Vliet*
1,2
,
Stefano De Tito*
1
,
Eugenia Almacellas*
1
,
Sharon A. Tooze
1
Molecular Cell Biology of Autophagy
,
The Francis Crick Institute
,
2
MRC Laboratory of Molecular Biology
Tags
HEK293A Cells
Endogenous Staining
ATG9A
Formaldehyde Fixation
Permeabilization
Digitonin
Blocking
Primary Antibody
Secondary Antibody
DAPI
Mounting
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