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Journal
/
Biology
/
Rat Intestinal Crypts Isolation and Intestinal Organoids Generation
/
Establishment and Passaging of Small Intestine Organoids
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Establishment and Passaging of Small Intestine Organoids
Establishment and Passaging of Small Intestine Organoids
Rat Intestinal Crypts Isolation and Intestinal Organoids Generation
DOI:
10.3791/200273-v
•
05:32 min
•
June 23, 2023
•
Eleanor Zagoren*
1
,
Anderson K. Santos*
1,2
,
Nadia A. Ameen
4
,
Kaelyn Sumigray
5,6
1
Department of Genetics
,
Yale School of Medicine
,
2
Department of Pediatrics
,
Yale School of Medicine
,
3
Department of Pediatrics/Gastroenterology and Hepatology
,
Yale School of Medicine
,
4
Department of Cellular and Molecular Physiology
,
Yale School of Medicine
,
5
Yale Stem Cell Center
,
Yale School of Medicine
,
6
Yale Cancer Center
,
Yale School of Medicine
Tags
Intestinal Organoids
Small Intestine
Rat
Dissection
Intestinal Fragment
Mesentery
PBS
EDTA
Epithelium
Villi
Crypts
Cell Culture
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