JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
Investigating Zinc Transport Mechanism in Mammalian Cells with FluoZin-3 Assay
/
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells
Investigating Zinc Transport Mechanism in Mammalian Cells with FluoZin-3 Assay
DOI:
10.3791/200360-v
•
04:55 min
•
June 02, 2023
•
Tomer Eli Ben Yosef
,
Raz Zarivach
,
Arie Moran
1
Department of Life Sciences and National Institute for Biotechnology in the Negev
,
Ben-Gurion University
,
2
Department of Physiology and Cell Biology, Faculty of Health Sciences
,
Ben-Gurion University
Tags
Sample Preparation
Fluorescence Measurement
Transfected HEK293T Cells
Microscope
Light Source
Camera
Suction System
Ringer’s Solution
Ringer’s Zinc Solution
Pyrithione
Perfusion Chamber
Sealing Silicone
Coverslip
Ringer’s Washing Solution
Perfusion Rate
Imaging Software
MCherry
ROIs
Background ROI
GFP
Perfect Focus System
PFS
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality
Zinc-Specific Staining of Transfected HEK293T Cells
Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells
Read Article