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Journal
/
Biology
/
Assessment of
Acinetobacter
Biofilm Formation and Characterization of Biofilms
/
用于
不动杆菌
生物膜形成的细菌接种物的制备
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of Bacterial Inoculum for
Acinetobacter
Biofilm Formation
Please note that all translations are automatically generated.
Click here for the English version.
用于
不动杆菌
生物膜形成的细菌接种物的制备
Assessment of
Acinetobacter
Biofilm Formation and Characterization of Biofilms
DOI:
10.3791/200399-v
•
01:38 min
•
August 04, 2023
•
Joo-Sung Kim
2
,
Jihoon Lim
1
Korea Food Research Institute
,
2
Department of Food Biotechnology
,
Korea University of Science and Technology
,
3
Advanced Radiation Technology Institute
,
Korea Atomic Energy Research Institute
Tags
Bacterial Inoculum Preparation For Acinetobacter Biofilm Formation: 1. Inoculate A Blood Agar Plate With 2-10 μL Of The Bacterial Strain Using A Sterile Pipette Tip. Streak The Agar Surface
Thinning It Out With Intermittent Flaming. Incubate The Plate At 30°C For 20-24 Hours. 2. Select A Single Colony From The Agar Plate And Inoculate 5 ML Of Sterile BHI Broth. Incubate The Inoculated Broth In A Shaking Incubator At 30°C
150 Rpm For 20-24 Hours. 3. Transfer 1 ML Of The Culture To A Sterile Microcentrifuge Tube And Centrifuge At 6
000 G For 10 Minutes At Room Temperature. Discard The Supernatant. 4. Add 1 ML Of Sterile Phosphate-buffered Saline (PBS) To The Pellet And Resuspend Using A Vortex. Centrifuge At 6
000 G For 10 Minutes At Room Temperature And Discard The Supernatant. 5. Resuspend The Resulting Pellet In Sterile
10-fold Diluted BHI Broth Using A Vortex. Ensure The Optical Density Is Around 0.1 At 600 Nm
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