JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
Isolation of Mitochondrial Contact Sites
/
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
Isolation of Mitochondrial Contact Sites
DOI:
10.3791/200432-v
•
05:03 min
•
June 16, 2023
•
Siavash Khosravi*
1,2
,
Johanna Frickel*
1
,
Max E. Harner
1
Department of Cell Biology, Biomedical Center, Medical Faculty
,
Ludwig-Maximilians University Munich
,
2
Cardio-Metabolic Diseases
,
Boehringer Ingelheim Pharma GmbH & Co. KG
Tags
– Submitochondrial Vesicles- Mitochondrial Contact Site Proteins- Centrifugation- Sucrose Cushion- Sucrose Gradient- Trichloroacetic Acid Precipitation- Protein Analysis- Mitochondria- Yeast- Homogenization- Refractometer- MOPS Buffer- EDTA- PMSF- Protease Inhibitors
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
Generating Submitochondrial Vesicles from Saccharomyces cerevisiae Mitochondria to Isolate Mitochondrial Contact Sites
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
Read Article