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Journal
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Biology
/
Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
/
用于成像的酵母细胞的制备、成像条件的优化和图像采集
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection
Please note that all translations are automatically generated.
Click here for the English version.
用于成像的酵母细胞的制备、成像条件的优化和图像采集
Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
DOI:
10.3791/200471-v
•
02:33 min
•
June 02, 2023
•
Emily Jie-Ning Yang
,
Istvan R. Boldogh
2
,
Haojie Ji
,
Liza Pon
2
,
Theresa C. Swayne
2
1
Department of Pathology and Cell Biology
,
Columbia University Irving Medical Center
,
2
Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center
,
Columbia University Irving Medical Center
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Keywords: Yeast Cells
Mitochondria
HyPer7
Microscopy
Imaging
Fluorescence
Confocal Microscopy
SCMOS Camera
Binning
Laser Power
Exposure
Dynamic Range
Noise Level
Time-lapse
Z-stacks
Transmitted Light
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