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Journal
/
Biology
/
Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
/
Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts (Video) | JoVE
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts
Semiautomated Analysis of mtHyPer7 in Yeast Cells with Scripts (Video) | JoVE
Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
DOI:
10.3791/200472-v
•
05:11 min
•
June 02, 2023
•
Emily Jie-Ning Yang
,
Istvan R. Boldogh
2
,
Haojie Ji
,
Liza Pon
2
,
Theresa C. Swayne
2
1
Department of Pathology and Cell Biology
,
Columbia University Irving Medical Center
,
2
Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center
,
Columbia University Irving Medical Center
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Keywords: MtHyPer7
Yeast Cells
Semiautomated Analysis
Fiji
Biosensor Analysis Script
Ratio Calculation
Background Subtraction
Noise Subtraction
Thresholding
Regions Of Interest
ROI Manager
Multichannel Z Stack Image
Measurement Table
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