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Journal
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Cancer Research
/
FISH for Long Non-Coding RNA Localization in Human Osteosarcoma Cells
/
RNA Fluorescence In Situ Hybridization to Localize the lncRNAs in Human Osteosarcoma Cells (Video) | JoVE
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
RNA Fluorescence In Situ Hybridization to Localize the lncRNAs in Human Osteosarcoma Cells
RNA Fluorescence In Situ Hybridization to Localize the lncRNAs in Human Osteosarcoma Cells (Video) | JoVE
FISH for Long Non-Coding RNA Localization in Human Osteosarcoma Cells
DOI:
10.3791/200526-v
•
04:42 min
•
June 16, 2023
•
Junli Chang*
1,2
,
Xiaoping Ma*
1,2
,
Xingyuan Sun
2
,
Chujie Zhou
2
,
Peng Zhao
2
,
Yongjun Wang
2
,
Yanping Yang
2
1
Longhua Hospital
,
Shanghai University of Traditional Chinese Medicine
,
2
Key Laboratory of Theory and Therapy of Muscles and Bones
,
Ministry of Education
Tags
Keywords: RNA Fluorescence In Situ Hybridization
LncRNA Localization
143B Osteosarcoma Cells
Hybridization Buffer
Probe Mix
Cell Fixation
Permeabilization
Ethanol Dehydration
Probe Hybridization
Wash Buffers
DAPI Staining
Mounting
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