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Journal
/
Biology
/
Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells
/
Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting
JoVE Journal
Biology
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JoVE Journal
Biology
Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting
Mesenchymal Stem Cell Sample Preparation, Flow Cytometric Analysis, and Cell Sorting
Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells
DOI:
10.3791/200704-v
•
05:25 min
•
November 10, 2023
•
Ayona Gupta*
1
,
Risani Mukhopadhyay*
1
,
Himanshi Khandelwal
,
Narendra Nala
,
Uttara Chakraborty
1
Manipal Institute of Regenerative Medicine, Bengaluru
,
Manipal Academy of Higher Education, Manipal
,
2
HIV-AIDS Laboratory, Molecular Biology and Genetics Unit
,
Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)
Tags
Mesenchymal Stem Cell
Flow Cytometric Analysis
Cell Sorting
Sample Preparation
Fax Tubes
Cell And Antibody Suspension
Staining Buffer
FBS
10% MSC Media
DAPI
Compensation Matrix
Single Stain Compensation Tubes
Acquisition Dashboard
Gating Strategy
Collection Plate
Single Cell Sort Purity Mask
Collection Device
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