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High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM

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Préparation de coupes de cerveau humain fixées au formol pour la technique de transformation tissulaire SHORT

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Preparing Formalin-Fixed Human Brain Sections for SHORT Tissue Transformation Technique

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Préparation de coupes de cerveau humain fixées au formol pour la technique de transformation tissulaire SHORT

High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM

DOI:

10.3791/200805-v

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00:47 min

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January 26, 2024

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Danila Di Meo*¹, Josephine Ramazzotti*¹, Marina Scardigli*^1,2, Franco Cheli, Luca Pesce³, Niamh Brady, Giacomo Mazzamuto^4,5, Irene Costantini^4,6, Francesco S. Pavone^4,5

¹European Laboratory for Non-linear Spectroscopy (LENS),University of Florence, ²Division of Physiology, Department of Experimental and Clinical Medicine,University of Florence, ³Department of Physics,University of Pisa, ⁴National Research Council – National Institute of Optics (CNR-INO), ⁵Department of Physics and Astronomy,University of Florence, ⁶Department of Biology,University of Florence

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Formalin-fixed Human Brain Agarose PBS Vibratome Preservation Solution Sodium Azide

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