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Journal
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Neuroscience
/
High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM
/
SWITCH - H
2
O
2
- Recupero dell'antigene — TDE (SHORT) Tecnica di trasformazione tissutale
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
SWITCH – H
2
O
2
– Antigen Retrieval — TDE (SHORT) Tissue Transformation Technique
Please note that all translations are automatically generated.
Click here for the English version.
SWITCH - H
2
O
2
- Recupero dell'antigene — TDE (SHORT) Tecnica di trasformazione tissutale
High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM
DOI:
10.3791/200806-v
•
02:57 min
•
January 26, 2024
•
Danila Di Meo*
1
,
Josephine Ramazzotti*
1
,
Marina Scardigli*
1,2
,
Franco Cheli
,
Luca Pesce
3
,
Niamh Brady
,
Giacomo Mazzamuto
4,5
,
Irene Costantini
4,6
,
Francesco S. Pavone
4,5
1
European Laboratory for Non-linear Spectroscopy (LENS)
,
University of Florence
,
2
Division of Physiology, Department of Experimental and Clinical Medicine
,
University of Florence
,
3
Department of Physics
,
University of Pisa
,
4
National Research Council – National Institute of Optics (CNR-INO)
,
5
Department of Physics and Astronomy
,
University of Florence
,
6
Department of Biology
,
University of Florence
Tags
Antigen Retrieval
SWITCH
H2O2
TDE
Formalin-fixed
Paraffin-embedded
Brain Sections
Switch-off Solution
Switch-on Solution
PBS
Inactivation Solution
Clearing Solution
PBST
Hydrogen Peroxide
Antigen Retrieval Solution
Primary Antibody
Secondary Antibody
Refractive Index Matching
TDE
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