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Journal
/
Biology
/
Transcriptome Profiling for High-Throughput Drug Screening
/
CDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing
JoVE Journal
Biology
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JoVE Journal
Biology
cDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing
CDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing
Transcriptome Profiling for High-Throughput Drug Screening
DOI:
10.3791/200935-v
•
02:34 min
•
February 23, 2024
•
Jacqueline Leidner
2
,
Heidi Theis
,
Michael Kraut
,
Alice Ragogna
,
Marc Beyer
3,4
,
Joachim Schultze
2,3
,
Jonas Schulte-Schrepping
2
,
Caterina Carraro
2
,
Lorenzo Bonaguro
2
1
Systems Medicine
,
German Center for Neurodegenerative Diseases (DZNE) eV.
,
2
Genomics & Immunoregulation, LIMES Institute
,
University of Bonn
,
3
PRECISE Platform for Single Cell Genomics and Epigenomics
,
DZNE and University of Bonn
,
4
Immunogenomics & Neurodegeneration
,
German Center for Neurodegenerative Diseases (DZNE) eV.
Tags
CDNA Library Preparation
Drug-treated PBMC Lysate
MRNA Sequencing
Reverse Transcription
Pre-amplification
CDNA Cleanup
Magnetic Purification Beads
Tagmentation
Enrichment PCR
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