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Transcriptome Profiling for High-Throughput Drug Screening

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cDNA-bibliotheekvoorbereiding van het met geneesmiddelen behandelde lysaat van perifere mononucleaire bloedcellen (PBMC) voor mRNA-sequencing

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JoVE Journal Biology

cDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing

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cDNA-bibliotheekvoorbereiding van het met geneesmiddelen behandelde lysaat van perifere mononucleaire bloedcellen (PBMC) voor mRNA-sequencing

Transcriptome Profiling for High-Throughput Drug Screening

DOI:

10.3791/200935-v

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02:34 min

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February 23, 2024

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Jacqueline Leidner², Heidi Theis, Michael Kraut, Alice Ragogna, Marc Beyer^3,4, Joachim Schultze^2,3, Jonas Schulte-Schrepping², Caterina Carraro², Lorenzo Bonaguro²

¹Systems Medicine,German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute,University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics,DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration,German Center for Neurodegenerative Diseases (DZNE) eV.

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Keywords: CDNA Library Preparation Drug-treated PBMC Lysate MRNA Sequencing Reverse Transcription Pre-amplification CDNA Cleanup Magnetic Purification Beads Tagmentation Enrichment PCR

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