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Journal
/
Bioengineering
/
Semi-Automated Mechanical-Enzymatic Tissue Dissociation for Single-Cell Analysis
/
Semi-Automated Mechanical Dissociation of Mouse Tissues to Generate Single-Cell Suspensions
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Semi-Automated Mechanical Dissociation of Mouse Tissues to Generate Single-Cell Suspensions
Semi-Automated Mechanical Dissociation of Mouse Tissues to Generate Single-Cell Suspensions
Semi-Automated Mechanical-Enzymatic Tissue Dissociation for Single-Cell Analysis
DOI:
10.3791/200997-v
•
03:27 min
•
November 10, 2023
•
Mayowa Amosu
,
Andrew J. Gregory
,
John D. Murtagh
,
Nitay Pavin
,
Carson Taylor Meyers
,
Juan Grano de Oro Fernandez
,
Kaitlyn Moore
,
Katharina Maisel
1
Fischell Department of Bioengineering
,
University of Maryland
,
2
UMD Terrapin Works
,
University of Maryland
Tags
Single-cell Suspension
Semi-automated Mechanical Dissociation
C-tube
Motorized Device
Custom Program
Digestion Enzyme
EDTA
Cell Strainer
Red Blood Cell Lysis
Centrifugation
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