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Journal
/
Biology
/
Profiling the Bacterial Community in Grapes Using Amplicon Metagenomics
/
High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity
JoVE Journal
Biology
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JoVE Journal
Biology
High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity
High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity
Profiling the Bacterial Community in Grapes Using Amplicon Metagenomics
DOI:
10.3791/201076-v
•
04:21 min
•
December 01, 2023
•
Adrienne Goppold
,
Louis Conradie
,
Otávio Guilherme Gonçalves de Almeida
,
Elaine Cristina Pereira De Martinis
,
Folarin Anthony Oguntoyinbo
1
A.R. Smith Department of Chemistry and Fermentation Sciences
,
Appalachian State University
,
2
Faculdade de Ciências Farmacêuticas de Ribeirão Preto
,
Universidade de São Paulo
Tags
Keywords: 16S RRNA Gene
V4 Hypervariable Region
PCR
Sequencing
Adapter Ligation
Magnetic Beads
DNA Concentration
Fluorimeter
FASTQ Files
Mapping File
QIIME2
DADA2
Naive Bayes Classifier
Silva Database
OTU
Taxonomic Assignment
MicrobiomeDB
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