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测量细菌细胞的抗弯刚度，使用光学陷阱

JoVE Journal
Biology

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JoVE Journal Biology

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

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测量细菌细胞的抗弯刚度，使用光学陷阱

DOI:

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05:45 min

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April 26, 2010

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Siyuan Wang, Hugo Arellano-Santoyo, Peter A. Combs, Joshua W. Shaevitz

¹Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics,Princeton University, ²Department of Physics, Lewis-Sigler Institute for Integrative Genomics,Princeton University

Chapters

00:00Title
00:41Introduction
01:12Attaching Bacteria to a Coverlip and Preparing the Beads
03:19Measuring the Bending Stiffness of the Bacterial Cells
05:11Measuring the Bending Stiffness of Bacterial Cells Representative Results
05:21Conclusion
05:44Credits

Summary

Automatic Translation

我们提出了一个封面防滑表面附有一个光学陷阱来衡量细胞的抗弯刚度弯曲的丝状细菌细胞的协议。

Tags

Measuring Bending Stiffness Bacterial Cells Optical Trap Protocol Filamentous Rod-shaped Bacteria Forces Microscopic Three-dimensional Spring High-intensity Laser Beam Chemically-treated Coverslip Live Bacteria Growing Ends Filamentous Growth Drug Cephalexin Cell Attachment Bending Forces Polylysine-coated Bead Force Measurement Displacement Measurement

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