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Development of a Click-Based Assay for Screening HAT1 Enzyme Activity

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Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Development of a Click-Based Assay for Screening HAT1 Enzyme Activity

DOI:

10.3791/201342-v

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04:18 min

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January 26, 2024

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Shreenidhi Rajkumar, Danielle Dixon, Andrew M. Lipchik, Joshua J. Gruber

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

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Keywords: Click Assay Histone Acetyltransferase 1 Acetylation Biotinylated Histone H4 Peptide Acetylation Reactions 4-Penten Oil Co-enzyme A BSA Pre-blocked Black NeutrAvidin-coated 96-well Plates THPTA Copper Mixture Sodium Ascorbate Biotin Azide Streptavidin Horseradish Peroxidase Amplex Red Detection Reagents Hydrogen Peroxide Sodium Phosphate

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