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Journal
/
Biology
/
Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation
/
Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation
JoVE Journal
Biology
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JoVE Journal
Biology
Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation
Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation
Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation
DOI:
10.3791/201503-v
•
01:14 min
•
January 05, 2024
•
Hiroko Shinjo
,
Gaku Nagano
,
Shogo Ishii
,
Natsumi Himeno
,
Yutaro Yamamoto
,
Junji Sagawa
,
Ryuta Baba
,
Gentaro Egusa
,
Noboru Hattori
,
Haruya Ohno
1
Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences
,
Hiroshima University
Tags
Cell Lysis
HEK293 Cells
Transfection
Protein Sample Preparation
Immunoprecipitation
Protein Lysis Buffer
Protease Inhibitors
Cell Scraper
Centrifugation
Protein Concentration
Protein Volume Adjustment
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