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Journal
/
Immunology and Infection
/
Elucidating T-Cell Kinetics: Methods for Real-Time Migration Analysis
/
Tracking Migration of Activated T-Cells Isolated from Mouse Secondary Lymphoid Tissues
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Tracking Migration of Activated T-Cells Isolated from Mouse Secondary Lymphoid Tissues
Tracking Migration of Activated T-Cells Isolated from Mouse Secondary Lymphoid Tissues
Elucidating T-Cell Kinetics: Methods for Real-Time Migration Analysis
DOI:
10.3791/201599-v
•
02:05 min
•
May 24, 2024
•
Allison T. Ryan
2
,
Ankit Dahal
2
,
Sana Mir
2
,
Minsoo Kim
2
,
Kihong Lim
2
1
Department of Microbiology and Immunology
,
University of Rochester
,
2
David H. Smith Center for Vaccine Biology and Immunology
,
University of Rochester
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T-cell Migration
Live Cell Imaging
Time-lapse Microscopy
Velocity Software
Contrast Enhancement
Object Finding
Cell Size Exclusion
Track Joining
Manual Tracking
ImageJ
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