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Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

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Production d’AAV recombinant par transfection transitoire

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Production of Recombinant AAV by Transient Transfection

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Production d’AAV recombinant par transfection transitoire

Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

DOI:

10.3791/201698-v

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02:38 min

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April 05, 2024

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Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^2,4, Carina Henriques^2,5, Ana C. Silva^2,3, Diana D. Lobo^2,3, Luísa Cortes^2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC – Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB – Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC – Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector – Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC – Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC – Microscopy Imaging Center of Coimbra – CNC,University of Coimbra

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Keywords: Recombinant AAV Transient Transfection HEK293T Cells Cell Culture Cell Transfection Polyethyleneimine DMEM Cell Harvesting Cell Lysis Benzonase Nuclease Centrifugation Filtration

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