1CNC-UC – Center for Neuroscience and Cell Biology,University of Coimbra, 2CIBB – Center for Innovative Biomedicine and Biotechnology,University of Coimbra, 3IIIUC – Institute for Interdisciplinary Research,University of Coimbra, 4ViraVector – Viral Vectors for Gene Transfer Core Facility,University of Coimbra, 5FFUC – Faculty of Pharmacy,University of Coimbra, 6MICC-CNC – Microscopy Imaging Center of Coimbra – CNC,University of Coimbra
This manuscript describes a detailed protocol for the generation and purification of adeno-associated viral vectors using an optimized heparin-based affinity chromatography method. It presents a simple, scalable, and cost-effective approach, eliminating the need for ultracentrifugation. The resulting vectors exhibit high purity and biological activity, proving their value in preclinical studies.
