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Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

April 5th, 2024
*1,2,3, *1,2,3, 1,2,4, 1,2,5, 1,2,3, 1,2,3, 1,2,3,6, *1,2,4,5, *1,2,3,4
1CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, 2CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3IIIUC - Institute for Interdisciplinary Research, University of Coimbra, 4ViraVector - Viral Vectors for Gene Transfer Core Facility, University of Coimbra, 5FFUC - Faculty of Pharmacy, University of Coimbra, 6MICC-CNC - Microscopy Imaging Center of Coimbra - CNC, University of Coimbra
* These authors contributed equally

Summary
Automatic Translation

该手稿描述了使用优化的基于肝素的亲和色谱方法生成和纯化腺相关病毒载体的详细方案。它提供了一种简单、可扩展且具有成本效益的方法，无需超速离心。所得载体表现出高纯度和生物活性，证明了它们在临床前研究中的价值。

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