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Journal
/
Bioengineering
/
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
/
क्षणिक अभिकर्मक द्वारा पुनः संयोजक एएवी का उत्पादन
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Production of Recombinant AAV by Transient Transfection
Please note that all translations are automatically generated.
Click here for the English version.
क्षणिक अभिकर्मक द्वारा पुनः संयोजक एएवी का उत्पादन
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
DOI:
10.3791/201698-v
•
02:38 min
•
April 05, 2024
•
Miguel M. Lopes*
1,2,3
,
Sara M. Lopes*
1,2,3
,
Rafael Baganha
2,4
,
Carina Henriques
2,5
,
Ana C. Silva
2,3
,
Diana D. Lobo
2,3
,
Luísa Cortes
2,3,6
,
Luís Pereira de Almeida*
1,2,4,5
,
Rui Jorge Nobre*
1,2,3,4
1
CNC-UC – Center for Neuroscience and Cell Biology
,
University of Coimbra
,
2
CIBB – Center for Innovative Biomedicine and Biotechnology
,
University of Coimbra
,
3
IIIUC – Institute for Interdisciplinary Research
,
University of Coimbra
,
4
ViraVector – Viral Vectors for Gene Transfer Core Facility
,
University of Coimbra
,
5
FFUC – Faculty of Pharmacy
,
University of Coimbra
,
6
MICC-CNC – Microscopy Imaging Center of Coimbra – CNC
,
University of Coimbra
Tags
Recombinant AAV
Transient Transfection
HEK293T Cells
Cell Culture
Cell Transfection
Polyethyleneimine
DMEM
Cell Scraper
PBS
Cell Pellet
Sodium Deoxycholate
Benzonase Nuclease
Cell Lysis
Centrifugation
Filtration
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