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Journal
/
Bioengineering
/
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
/
FPLC-Aufreinigung und Charakterisierung von rekombinanten AAV
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
FPLC Purification and Characterization of Recombinant AAV
Please note that all translations are automatically generated.
Click here for the English version.
FPLC-Aufreinigung und Charakterisierung von rekombinanten AAV
Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography
DOI:
10.3791/201699-v
•
04:45 min
•
April 05, 2024
•
Miguel M. Lopes*
1,2,3
,
Sara M. Lopes*
1,2,3
,
Rafael Baganha
2,4
,
Carina Henriques
2,5
,
Ana C. Silva
2,3
,
Diana D. Lobo
2,3
,
Luísa Cortes
2,3,6
,
Luís Pereira de Almeida*
1,2,4,5
,
Rui Jorge Nobre*
1,2,3,4
1
CNC-UC – Center for Neuroscience and Cell Biology
,
University of Coimbra
,
2
CIBB – Center for Innovative Biomedicine and Biotechnology
,
University of Coimbra
,
3
IIIUC – Institute for Interdisciplinary Research
,
University of Coimbra
,
4
ViraVector – Viral Vectors for Gene Transfer Core Facility
,
University of Coimbra
,
5
FFUC – Faculty of Pharmacy
,
University of Coimbra
,
6
MICC-CNC – Microscopy Imaging Center of Coimbra – CNC
,
University of Coimbra
Tags
FPLC Purification
Recombinant AAV
Heparin Column
Buffer A
Buffer B
Sample Application
Column Equilibration
Sample Elution
Fraction Collection
Buffer Exchange
Concentration
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