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Immunology and Infection

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胞内相互作用的可视化和量化脑膜炎奈瑟菌和人类α-辅肌动蛋白共聚焦成像

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

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胞内相互作用的可视化和量化脑膜炎奈瑟菌和人类α-辅肌动蛋白共聚焦成像

DOI:

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12:58 min

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October 24, 2010

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Isabel Murillo, Mumtaz Virji

¹Department of Cellular and Molecular Medicine,University of Bristol, UK

Chapters

00:00Title
01:03Introduction
01:39Preparation of Bacterial ( N. meningitidis) Suspension
03:09Seeding and Infection of Target Cells
04:29Immuno-staining
06:21Confocal Laser Scanning Microscopy
08:56Quantitative Colocalization Analysis
09:55Intracellular Localization of Opc-expressing N. meningitidis and Alpha-actinin
12:23Conclusion

Summary

Automatic Translation

脑膜炎奈瑟菌 （NM），革兰氏阴性人类特有的呼吸道病原体，人类α-辅肌动蛋白结合。在这里，我们提出了一个可视化的细菌colocalisation后细菌进入到人脑微血管内皮细胞（HBMECs）的α-辅肌动蛋白与细胞内的协议。

Tags

Neisseria Meningitidis α-actinin Confocal Imaging Intracellular Interactions Visualisation Quantification Opc Protein Adhesin Invasin Integrins Vitronectin Host Cell Proteins Electrophoresis Blotted Nitrocellulose Mass Spectrometry Protein Interaction Endothelial Cells

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