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在激活人类细胞中的基因表达的动态检测组蛋白修饰染色质免疫沉淀(ChIP)
JoVE Journal
Biology
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JoVE Journal Biology
Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
DOI:

13:20 min

July 29, 2010

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Chapters

  • 00:05Title
  • 01:11Introduction
  • 01:33Crosslinking
  • 03:32Preparing Chromatin
  • 05:37Chromatin IP (ChIP)
  • 07:50Collecting Immunocomplexes
  • 10:01Recovering the DNA
  • 11:45Representative Results
  • 12:44Conclusion

Summary

Automatic Translation

本协议描述了如何染色质免疫沉淀(ChIP)是用来研究的动态改变,调节信号转导通路诱导转录的染色质模板。

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