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Generation of Recombinant Influenza Virus from Plasmid DNA
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Recombinant Influenza Virus from Plasmid DNA

Generation of Recombinant Influenza Virus from Plasmid DNA

DOI:
10.3791/2057-v

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11:31 min

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August 03, 2010

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,
1Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, 2Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute,Mount Sinai School of Medicine

Chapters

  • 00:04Title
  • 01:00Introduction
  • 01:29Influenza Virus Rescue Transfection
  • 04:54Infecting MDCK Cells & TC Harvest
  • 06:14Egg Infection & Allonotic Fluid Harvest
  • 07:55HA Assay
  • 08:55Confirmation of Rescue
  • 10:19Conclusion

Summary

Automatic Translation

Automatic Translation

Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.

Tags

Recombinant Influenza VirusPlasmid DNAReverse GeneticsInfluenza ResearchInfectious VirusesViral ProteinsMutationsViral ReplicationPathogenesisViral VectorsForeign Proteins

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