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RhoC GTPasi attivazione di analisi
JoVE Journal
Biology
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JoVE Journal Biology
RhoC GTPase Activation Assay
DOI:

09:58 min

August 22, 2010

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Chapters

  • 00:00Title
  • 00:48Introduction
  • 01:25Preparation of GST-fusion Protein
  • 04:41GST-RBD Pull Down Assay
  • 09:01Representative Results for a RhoC GTPase Activation Assay
  • 09:34Conclusion

Summary

Automatic Translation

Questo protocollo utilizza un pull down di analisi per determinare i livelli di attivo GTPasi RhoC all'interno delle cellule.

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