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Single Cell Transfection in Chick Embryos
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Single Cell Transfection in Chick Embryos

Single Cell Transfection in Chick Embryos

DOI:
10.3791/2133-v

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08:46 min

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September 25, 2010

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1Department of Medical Neurobiology,Hadassah Medical School – Hebrew University

Chapters

  • 00:00Title
  • 00:57Introduction
  • 01:21Micropipette, DNA, and Embryo Preparation
  • 04:15Loading Micropipettes and Microinjection
  • 06:49Representative Injection Results
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

Using fine tip micropipettes we inject plasmid DNA into subdomains of chicken somites or neural tubes. The concentration of the plasmid is adjusted to generate single transfected cells. We then allow the cells to develop into clonal populations.

Tags

Single Cell TransfectionChick EmbryosDevelopmental BiologyLineage DiversificationMolecular MechanismsTransfection MethodsSingle ProgenitorsIn-ovo TransfectionLineage TracingGenetic ManipulationSomiteNeural TubeEpitheliumSporadic TransfectionClonal PopulationsReportersGenetic Manipulation ToolsElectroporation MethodIn-vivo Models

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