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Chapters
- 00:00Title
- 00:57Introduction
- 01:21Micropipette, DNA, and Embryo Preparation
- 04:15Loading Micropipettes and Microinjection
- 06:49Representative Injection Results
- 08:18Conclusion
Usando la punta fina micropipetas se inyecta el plásmido de ADN en subdominios de somitas pollo o tubos neurales. La concentración del plásmido se ajusta a generar solo las células transfectadas. A continuación, permitir que las células se desarrollan en poblaciones clonales.
Tags
Single Cell TransfectionChick EmbryosDevelopmental BiologyLineage DiversificationMolecular MechanismsTransfection MethodsSingle ProgenitorsIn-ovo TransfectionLineage TracingGenetic ManipulationSomiteNeural TubeEpitheliumSporadic TransfectionClonal PopulationsReportersGenetic Manipulation ToolsElectroporation MethodIn-vivo Models
