Journal
/
Immunology and Infection
/
Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis

Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis

DOI:
10.3791/2181-v

•

10:52 min

•

December 17, 2010

•

, , ,
1Department of Pathology,The Johns Hopkins University

Chapters

  • 00:05Title
  • 01:00Introduction
  • 03:21Collection and Storage of Mouse Pituitary Glands
  • 04:40Extraction of Mouse Pituitary Proteins
  • 07:00Preparation of the Emulsion
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

Autoimmune hypophysitis can be reproduced in mice by injecting an extract of mouse pituitary proteins.

Tags

Mouse Pituitary ImmunogenExperimental Autoimmune HypophysitisChronic InflammationPituitary GlandAutoimmunityRare DiseaseCancer PatientsInhibitory ReceptorsT LymphocytesCTLA-4PD-1 ReceptorsAdjuvantProtein ExtractionSJL Mice

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Induction of Experimental Autoimmune Hypophysitis in SJL Mice
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
check_url/2181?article_type=v&slug=preparation-mouse-pituitary-immunogen-for-induction-experimental

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

Read Article