A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Basic Science and Craniofacial Biology,College of Dentistry, New York University, 2Department of Internal Medicine and Department of Psychiatry,School of Medicine, Washington University in St. Louis, 3Veterans Affairs Medical Center, 4School of Dental Medicine, Department of Biochemistry,University of Pennsylvania-School of Medicine, 5Monell Chemical Senses Center, 6Monell Chemical Senses Center
Chapters
- 00:00Title
- 00:11Introduction
- 01:54Subject Preparation
- 03:39Tongue Papillae Removal Technique
- 06:54Representative Papillae Tissue Results
- 08:55Conclusion
Conoscenza dei meccanismi molecolari alla base di trasduzione gustativa ha recentemente goduto di significativi passi avanti, soprattutto grazie all'utilizzo di modelli animali. Tuttavia, la grande diversità di sensibilità e specificità tra gusto garantisce mammiferi studi nel tessuto umano. Descriviamo una tecnica di biopsia per raccogliere cellule gustative viventi dalle papille sulla lingua umana.
Tags
TechniqueCollectFungiform PapillaeHuman TongueSense Of TasteCriticalQuality Of FoodPotentially IngestedStimulate Metabolic ProcessesAlimentary CanalDigestionProgressUnderstandingBiochemical EventsMolecular EventsTaste TransductionAnimal ModelsReceptor SpecificityReceptor DensityBiopsy TechniqueRounded Pink Anterior StructuresDiameterTaste BudsIsolation Of Viable Taste Bud CellsIn Situ HybridizationImmunohistochemistryMolecular Biology TechniquesIdentification Of Taste-specific Novel Proteins
