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1Department of Basic Science and Craniofacial Biology,College of Dentistry, New York University, 2Department of Internal Medicine and Department of Psychiatry,School of Medicine, Washington University in St. Louis, 3Veterans Affairs Medical Center, 4School of Dental Medicine, Department of Biochemistry,University of Pennsylvania-School of Medicine, 5Monell Chemical Senses Center, 6Monell Chemical Senses Center
Chapters
- 00:00Title
- 00:11Introduction
- 01:54Subject Preparation
- 03:39Tongue Papillae Removal Technique
- 06:54Representative Papillae Tissue Results
- 08:55Conclusion
Conhecimento dos mecanismos moleculares subjacentes a transdução de gustativa tem apreciado recentemente avanços significativos, em grande parte devido ao uso de modelos animais. No entanto, a grande diversidade na sensibilidade gustativa e especificidade entre os estudos mamíferos garante no tecido humano. Descrevemos uma técnica de biópsia para coletar células gosto vivos de papilas na língua humana.
Tags
TechniqueCollectFungiform PapillaeHuman TongueSense Of TasteCriticalQuality Of FoodPotentially IngestedStimulate Metabolic ProcessesAlimentary CanalDigestionProgressUnderstandingBiochemical EventsMolecular EventsTaste TransductionAnimal ModelsReceptor SpecificityReceptor DensityBiopsy TechniqueRounded Pink Anterior StructuresDiameterTaste BudsIsolation Of Viable Taste Bud CellsIn Situ HybridizationImmunohistochemistryMolecular Biology TechniquesIdentification Of Taste-specific Novel Proteins
