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Preparation of Highly Coupled Rat Heart Mitochondria
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JoVE Journal Biology
Preparation of Highly Coupled Rat Heart Mitochondria

Preparation of Highly Coupled Rat Heart Mitochondria

DOI:
10.3791/2202-v

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12:28 min

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September 23, 2010

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,
1Faculty of Life Sciences,University of Manchester, 2School of Biological Sciences,Queen’s University Belfast

Chapters

  • 00:00Title
  • 00:07Introduction
  • 00:14General Scheme of the Protocol
  • 12:19Conclusion

Summary

Automatic Translation

Automatic Translation

We describe а protocol for isolation of pure, highly coupled rat heart mitochondria for functional or structural studies of cellular bioenergetics, biophysical measurements, proteomics or mitochondrial DNA and lipids analysis.

Tags

Keywords: MitochondriaHighly CoupledRat HeartOxidative PhosphorylationEnergy GenerationApoptosisSignalling OrganellesMammalian DevelopmentAgeingCell MetabolismCell ProliferationBiological ProcessesIsolationHandlingIntact MitochondriaLaboratory AnimalsCellular MetabolismKnock-out AnimalsRapid MethodFunctional ResearchStructural ResearchBioenergeticsBiomolecule TransportProteomic StudiesMitochondrial DNAProteinsLipids

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