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细胞的细胞大分子运输检测在植物利用基因枪法

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JoVE Journal Biology

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

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细胞的细胞大分子运输检测在植物利用基因枪法

DOI:

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07:14 min

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August 27, 2010

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Shoko Ueki, Benjamin L. Meyers, Farzana Yasmin, Vitaly Citovsky

¹Department of Biochemistry and Cell Biology,State University of New York at Stony Brook, ²Bio-Medical Engineering Department,NED University of Engineering and Technology

Chapters

00:00Title
00:51Introduction
01:24Target Plant Growth
02:32Biolistic Delivery of DNA-coasted Microparticles
04:09Imaging of Protein Expression
05:17Representative Results for a Cell-to-cell Macromelucular Transport Assay
06:38Conclusion

Summary

Automatic Translation

高分子植物细胞之间的贩运，可以通过瞬时表达利益荧光标记的蛋白质，分析其内部和细胞间共聚焦显微镜的分布进行评估。

Tags

Cell-to-cell Macromolecular Transport Assay Biolistic Bombardment Fluorescently Tagged Protein Encoding DNA Construct Confocal Microscopy Symplastic Protein Transport Arabidopsis Thaliana Nicotiana Benthamiana N. Tabacum (tobacco)

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