An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor
Transcript
Begin with an anesthetized mouse bearing a subcutaneous tumor.
In this tumor microenvironment, T-regulatory cells express an anti-cytotoxic protein that strongly interacts with CD80 on antigen-presenting cells or APCs, preventing conventional T cell interaction.
Disinfect the surgical area and make a skin incision near the tumor.
Open the wound to expose the tumor and trim it partially, leaving remnants of the tumor tissue.
Close the wound and allow the mouse to recover.
Post-surgery, take a syringe filled with antibodies specific to the anti-cytotoxic protein and intraperitoneally inject them into the anesthetized mouse for an adjuvant or additional therapy.
These antibodies travel to the tumor site and bind to anti-cytotoxic proteins on T-regulatory cells, blocking their function.
This enables APCs to interact with T cells, activating them to release cytotoxic molecules.
These molecules kill remaining tumor cells, potentially reducing tumor regrowth, suggesting effective adjuvant therapy.
