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Journal
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Immunology and Infection
/
胶粘剂基于磁带的采样和荧光组合
原位
杂交快速检测
沙门氏菌
新鲜农产品
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Combination of Adhesive-tape-based Sampling and Fluorescence
in situ
Hybridization for Rapid Detection of
Salmonella
on Fresh Produce
Please note that all translations are automatically generated.
Click here for the English version.
胶粘剂基于磁带的采样和荧光组合
原位
杂交快速检测
沙门氏菌
新鲜农产品
DOI:
10.3791/2308-v
•
09:10 min
•
October 18, 2010
•
Bledar Bisha
,
Byron F. Brehm-Stecher
1
Center for Meat Safety and Quality, Department of Animal Sciences
,
Colorado State University
,
2
Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition
,
Iowa State University
Chapters
00:00
Title
01:32
Introduction
02:07
Surface Sampling with Sterile Adhesive Tape
03:33
Solid-phase Enrichment
04:54
Fixation and Dehydration
05:35
Hybridization
06:46
Detection
07:43
FISH and FACS Analyses on Tomatoes
08:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了简单的基于磁带的粘合剂为番茄等新鲜农产品表面取样的方法,全细胞快速检测<em>沙门氏菌</em>用荧光<em>原位</em>杂交(FISH)。
Tags
Adhesive-tape-based Sampling
Fluorescence In Situ Hybridization
Rapid Detection
Salmonella
Fresh Produce
Culture-independent Detection
Cell-charged Tapes
Solid-phase Enrichment
Liquid Surface Miniculture
DNA Probe Cocktail
Hybridization
Fluorescence Microscopy
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