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监测后腹腔感染的免疫细胞贩运荧光朊病毒棒时间
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Immunology and Infection
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JoVE Journal Immunology and Infection
Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
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监测后腹腔感染的免疫细胞贩运荧光朊病毒棒时间

DOI:
10.3791/2349-v

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09:22 min

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November 19, 2010

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, , , , , ,
1Department of Microbiology, Immunology and Pathology,Colorado State University

Chapters

  • 00:00Title
  • 01:12Purifying and Labeling Prion Rods
  • 03:37Prion Inoculation and Cell Recovery
  • 06:51Cell Staining and Flow Cytometry
  • 08:09Cellular Trafficking Response to Purified Prion Rods
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一种新的分析监测朊蛋白的吸收和免疫细胞的活动，通过净化后立即腹腔注射和荧光标记从被感染的大脑，然后监测其吸收和运动从注射部位和调解这些事件的细胞特征的材料汇总朊病毒棒。

Tags

Immune CellsTraffickingFluorescent Prion RodsIntraperitoneal InfectionAbnormal FormHost-encoded Prion Protein (PrPC)Protease ResistantPathologicInfectiousPrion DiseasesChronic Wasting Disease (CWD)ScrapiePrion HypothesisImmune SystemPeripheral Prion PathogenesisComplement SystemDendritic CellsMacrophagesPrion UptakeTraffickingInoculation Site

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