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Western Blotting: Sample Preparation to Detection

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

DOI:

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07:45 min

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October 14, 2010

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Anna Eslami, Jesse Lujan

¹Research and Development,EMD Chemicals Inc.

Chapters

00:00Title
00:58Sample Preparation
01:52SDS-PAGE
03:21Protein Transfer
04:55Membrane Blocking and Detection
06:56Representative Western Blotting Results
07:26Conclusion

Summary

Automatic Translation

Western blotting is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract.

Tags

Western Blotting Sample Preparation Detection Proteins Extract Gel Electrophoresis Native Proteins Denatured Proteins Polypeptide Length 3-D Structure Membrane Transfer Nitrocellulose PVDF Antibody Detection

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