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Chapters
- 00:05Title
- 01:55Gel Electrophoresis with Stain-free Gels and Ge Imaging
- 03:29Protein Transfer with Trans-blot Turbo System and Antibody Incubation
- 06:32Imaging and Calculating the Total Protein Normalization: Image Lab
- 08:53Total Protein Normalization Results for MCM-7 in Irradiated Lymphoblastoid Cells
- 10:43Conclusion
V3 workflow is a western blot procedure using stain-free gels. The stain-free technology allows researchers to visualize protein separation quality, to verify the transfer efficiency, and most importantly, to validate the change in the protein of interest using total protein quantification as a reliable loading control.
