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从噬菌体抗体库的肽调节间质细胞的基因表达和使可能在Unicortical骨缺损的成骨

JoVE Journal
Biology

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JoVE Journal Biology

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Please note that all translations are automatically generated. Click here for the English version.

从噬菌体抗体库的肽调节间质细胞的基因表达和使可能在Unicortical骨缺损的成骨

DOI:

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07:53 min

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December 10, 2010

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Gary Balian, Gina Beck, Vedavathi Madhu, Robert Sikes, Quanjun Cui, Haixiang Liang, Joshua Bush

¹Orthopaedics Research,University of Virginia, ²Biological Sciences,University of Delaware, ³Orthopaedic Surgery,University of Virginia

Chapters

00:05Title
01:44Biopanning
03:39Peptide Staining of Mesenchymal Cultured Cells
04:42Preparation of Gel Foam Composite and In Vivo Implantation
05:25Histology
05:40Effects of Phage Display Peptides on Osteogenesis In Vitro and In Vivo
06:40Conclusion

Summary

Automatic Translation

噬菌体展示库，用于识别目标的骨肽序列。其目的是调查这些肽的间质细胞分化的影响，以确定其对骨再生的效果。

Tags

Peptides Phage Display Library Gene Expression Mesenchymal Cells Osteogenesis Unicortical Bone Defects Bone Formation Collagen Matrix Alkaline Phosphatase Activity Nodule Formation Runx2 Osterix Bone Sialoprotein Osteocalcin Collagen Sponge Repair Of Bone Defects In Vitro Differentiation In Vivo Delivery

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