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显微切割的黑寡妇蜘蛛丝绸生产腺体
JoVE Journal
Biology
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JoVE Journal Biology
Microdissection of Black Widow Spider Silk-producing Glands
DOI:

09:47 min

January 11, 2011

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Chapters

  • 00:05Title
  • 01:00Introduction
  • 01:57Preparation and Isolation of Spider Abdomen
  • 03:50Removal of Spider Abdominal Exoskeleton
  • 04:53Dissection and Isolation of Silk-Producing Glands
  • 07:42Evaluation of Gland mRNAs and Proteins Isolated through Spider Silk Gland Dissection
  • 08:43Conclusion

Summary

Automatic Translation

在这里,我们描述了一个有效的策略,以消除雌性黑寡妇蜘蛛腹部的丝生产腺体。此过程允许的七个不同的丝绸生产腺体高度纯化的时尚,一个调查研究蜘蛛丝的生产和纤维大会的重要的过程中迅速隔离。

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