/
/
Isolamento ed espansione della cellule staminali neurali del mouse utilizzando il saggio neurosfere
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:41Introduction
- 01:04Animal Dissection
- 02:25Brain Dissection
- 04:31Tissue Preparation and Plating Cells
- 09:04Passaging Neurospheres
- 11:56Representative Results
- 12:55Conclusion
Questo protocollo video viene illustrato il metodo di analisi per generare neurosfere ed espandere le cellule staminali neurali adulte provenienti dalla regione periventricolare del mouse, e fornisce approfondimenti tecnici per garantire la si può raggiungere culture neurosfere riproducibile.
Tags
IsolationExpansionAdult MouseNeural Stem CellsNeurosphere AssayPutativeMurine BrainReynolds And WeissChemically Defined Serum-free Culture SystemGrowth Factor Responsive Precursor CellsEpidermal Growth FactorBasic Fibroblastic Growth FactorColoniesUndifferentiated CellsNeurospheresSubculturedPool Of Neural Stem CellsDifferentiateThree Major Cell TypesCNSNeuronsAstrocytesOligodendrocytesInvaluable ToolIn Vitro StudiesTherapeutic PurposesVideo DemonstrationPeriventricular RegionReproducible Neurosphere Cultures
