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罗丹明-肌动蛋白标记F -肌动蛋白在通透的神经生长锥刺完
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JoVE Journal Neuroscience
Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
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罗丹明-肌动蛋白标记F -肌动蛋白在通透的神经生长锥刺完

DOI:
10.3791/2409-v

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09:14 min

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March 17, 2011

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1Department of Neuroscience,University of Minnesota

Chapters

  • 00:05Title
  • 01:49Preparation of Coverslips or “Video” Dishes
  • 03:40Preparation of Neuronal Cultures and Media
  • 04:53Permeabilization of Neuronal Cultures and Incorporation of Rhodamine-actin onto F-actin Barbed Ends
  • 06:13Variations to this Procedure
  • 07:35Representative Actin Labeling Results
  • 08:47Conclusion

Summary

Automatic Translation

Automatic Translation

描述的方法来可视化和量化，F -肌动蛋白在神经生长锥刺完。盖玻片上培养神经细胞后，细胞透用含皂甙的解决方案。然后，潜伏期短皂苷含有若丹明-肌动蛋白的缓冲区纳入到免费的肌动蛋白刺完肌动蛋白的荧光。

Tags

F-actinBarbed EndsRhodamine-actinPermeabilizedNeuronal Growth ConesAxonsMolecular Guidance CuesGrowth Cone CytoskeletonActin Filament MeshworkActin PolymerizationDynamic RemodelingGuidance CuesActin MonomerPolymerization NucleiChick RetinalDorsal Root Ganglion (DRG)Actin Depolymerizing Factor (ADF/cofilin)

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