/
/
Dispositivo microfluídicos para Recriando um microambiente do tumor<em> In Vitro</em
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:50Introduction
- 02:52Microfluidic Device Fabrication
- 05:45Formation of Uniform Tumor Spheroids using the Hanging Drop Method
- 07:04Introduction of Spheroids into the Microfluidic Device
- 09:18Introduction of Apoptosis-detecting Agent into the Microfluidic Device
- 10:50Introduction of Therapeutic Agents into the Microfluidic Device
- 11:59Time Lapse Microscopy and Estimation of Drug Diffusivity Coefficients
- 14:25Representative Results
- 15:30Concluding Remarks
Nós apresentamos o procedimento para a fabricação e operação de um dispositivo micro que recria heterogêneos microambientes tumorais<em> In vitro</em>. A variabilidade na apoptose dentro do tecido do tumor foi quantificada usando manchas fluorescentes e do coeficiente de difusão eficaz da doxorrubicina droga quimioterapêutica para o tecido do tumor foi avaliada.
Tags
Microfluidic DeviceTumor MicroenvironmentIn VitroDrug DeliverySystemic ClearanceHeterogeneousVasculatureNutrientsViable CellsQuiescent CellsNecrotic CellsCancer DrugsPenetrationMonolayersIn Vitro ModelPDMSSoft LithographyMulticellular Tumor SpheroidsHanging Drop MethodRectangular ChambersContinuous Medium PerfusionLinear GradientsFluorescent StainsApoptosis VariabilityChemotherapeutic Drug DoxorubicinDiffusion CoefficientCell Lines
