This content is Free Access.
Chapters
- 00:05Title
- 01:23Introduction
- 01:49Setting Up
- 02:33Fixing Flies in Collars
- 03:12Paraffin Sections of Drosophila Thoraxes
- 04:38Cryosections of Drosophila Thoraxes
- 05:39Lipid Detection in Drosophila Muscles
- 06:18Thoracic Muscles of Drosophila
- 07:08Conclusion
Identificatie van mechanismen die ten grondslag liggen aan spierschade is cruciaal. Hier presenteren we de histologische techniek voor het bereiden van in paraffine ingebedde en bevroren delen van Drosophila thoracale spieren. Dit maakt analyse van de spier-morfologie en lokalisatie van eiwitten en andere spiercel componenten.
Tags
Paraffin-embedded SectionsFrozen SectionsDrosophila Adult MusclesMolecular CharacterizationMuscular DystrophiesMyopathiesMuscle DiseaseGenetic AnalysisMuscle SpecificationMuscle FormationMuscle FunctionModel SystemsMuscle PhysiologyMuscle DamageDrosophila Multi-fiber MusclesVertebrate Striated MusclesDystrophic Muscle MorphologyMuscular FunctionAgeing Adult FliesHistological TechniqueStainingProtein Detecting DyesCryosectionsImmunohistochemical DetectionMorphological DefectsExpression PatternMuscle/neuron-specific Proteins
