A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:15Muscle Isolation/Homogenization and Mitochondrial Isolation
- 04:13Electrode Calibration
- 06:17Mitochondrial Respiration
- 08:15Representative Mitochondrial Respiration Results
- 09:15Conlcusion
Detta protokoll beskriver en procedur för att studera andning i mitokondrier isolerade från skelettmuskulaturen. Denna metod anpassades från Scorrano<em> Et al.</em> (2007). Den mitokondriella Isoleringsförfarande kräver ca 2 timmar. Den mitokondriella andning kan fyllas i ca 1 timme.
Tags
Mitochondrial IsolationSkeletal MuscleOrganellesMetabolic ReactionsATP SynthesisSignaling CascadesLiverBrainHeartSkeletal Muscle FunctionIsolation MethodBuffersReagentsMitochondrial Protein ConcentrationFunctional StudiesMitochondrial RespirationPolarographyClark-type ElectrodeOxygen Electrode CalibrationExperimental BufferGlutamateMalateAdenosine Diphosphate (ADP)Oligomycin
